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Performing 1D Analysis
61
Find Lanes and Bands: Searches for
lanes and bands in the image.
Edit Objects: Select, move and resize
lanes, bands, Rf lines, and the Region
of interest (ROI).
No Background Correction: Corrects
the background with methods: No
Correction, Straight Line, Joined
Valleys, Rolling Disc and Area Between
Lines.
Lane Profile Graph: Displays a line
graph of intensity or concentration value
verses position in the lane for the lane
or lanes selected.
Molecular Weight: Calibrates molecular
weight with Retardation factor (Rf) lines
and by applying standards to lanes.
Dendrogram: Compares the statistics
of bands in one lane to bands in other
lane for a single image.
Multi-Image Dendrogram: Compares
the statistics of bands in one lane to
bands in other lane for multiple images.
Data Explorer: Brings up datasheets
showing the results of calculations on
lanes and bands.
Delete all analysis data: Deletes all
recorded analysis data.
Lane Tools: View Lane Properties, Add
lane(s), Delete selected lane(s), Find
bands in selected lane(s), Straighten
selected lane(s), Auto curve selected
lane(s).
Band Tools: View Band properties,
Add bands(s), Delete selected band(s)
Rf Line Tools: Add Rf line, Delete
selected Rf line(s), Delete all Rf lines,
Select standard lane
Settings: Sets image analysis settings.
- Web site: uvp.com 1
- Table of Contents 2
- Introduction 6
- Minimum System Requirements 7
- On the Fly Activation 8
- User Administration 9
- Single-User License 9
- Usernames and Passwords 10
- Technical Support 12
- License Agreement 13
- Limitation of Liability 14
- Navigating the Software 15
- Toolbars 16
- Image Windows 18
- Status Bar 19
- Using Plug-In Modules 20
- Positioning Plug-in Modules 21
- Histogram Controls 22
- Digital Video Player 22
- Using the Player 23
- Player Features 23
- Player Options 24
- Image Controls Plug-In 26
- Change Contrast 27
- Change Gamma 27
- 3D Plots 28
- FTP Transfer 29
- Log Viewer 29
- Zoom/Pan 29
- Using Zoom In or Out 30
- Magnify Part of an Image 30
- Camera Plug-In 32
- Capturing Images 35
- Creating Templates 36
- Creating Colony Templates 37
- Editing Images 39
- Undo and Redo 40
- Using Image Filters 42
- Background Filter 43
- Burn Changes to a New Image 44
- Convert Image 44
- Duplicating Images 44
- Flip Image 44
- Image Enhancement Filters 45
- Image History 46
- Measurement Tools 47
- Merging Two Images 48
- Obtaining Image Information 49
- Pseudocolor 50
- Reduce Image to Mono 51
- Resize Image 51
- Rotate and Align Image 52
- Rulers 53
- Scanning Images 53
- Spatial Calibration 54
- Annotations 56
- New Annotation Window 57
- Selecting Annotations 58
- Formatting Annotations 60
- Deleting Annotations 60
- Creating Annotations 61
- Loading and Saving Images 62
- Image File Types 63
- The Extended Attributes File 64
- Performing 1D Analysis 65
- Finding Lanes and Bands 67
- 1D Analysis Settings 70
- Resize Band Extents 71
- Delete Lanes and Bands 72
- Lane and Band Information 73
- Lane Profile Graph 74
- Display Multiple Lanes 75
- Change the Selected Lane 75
- Axis Options 75
- Display Options 75
- Selecting Background Color 76
- Overview 77
- Filtering Data 77
- Export Data Explorer Reports 80
- Using the 1D Analysis Plugin 83
- Specific Correction Options 83
- Concentration Calibration 84
- Unit Type 85
- Molecular Weight Calibration 88
- Using the Stretch Factor 89
- Exact Placement of Bands 90
- Adding Rf Lines Manually 91
- Generating Dendrogram Graphs 93
- Modify Dendrogram Graphs 93
- Creating clusters 94
- Linkage rules 94
- Performing Colony Counting 96
- Label Type 97
- Colony Marking 97
- Label Color 97
- Automatic Counting 98
- Manual Counting 98
- Edit Colonies 100
- Add Colonies 100
- Delete Colonies 101
- Split Colonies 101
- Merging Colonies 102
- Spiral Counting 102
- Zone Analysis 103
- Reporting Functions 104
- Classes 104
- Statistics 105
- Distribution 105
- Printing Reports and Images 106
- Purpose 108
- Features 108
- Glossary 110
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