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Perform 1D Analysis
113
Apply a Molecular Weight Standard to a Lane
• Calibrate a Lane
• Stretch Factor
• Manual Placement of Weights
• Exact Placement of Bands
Calibrate a Lane
• First identify all lanes and bands in the image.
• Select the lane to calibrate. [ Edit Lanes and Bands ]
• From the 1D Analysis Action Tab > Lane Profile Menu select Calibrate Lane.
• A new Molecular Weight window opens. On tab Step 1: Select MW standard click on the
standard to use and then select Next. If the standard is not on the list, click Add to add a
standard to the list (see Adding a Molecular Weight Standard to the Library).
- Life Science Software 1
- Table of Contents 3
- LS Software User Guide 13
- Welcome 15
- Capturing Images 16
- Minimum System Requirements 17
- Registering the Software 18
- Offline Activation 19
- Single-User License 21
- Network-User License 22
- Additional Network Users 26
- User Administration 28
- Users Rights 30
- Configure User Accounts 31
- Add a New User 32
- Edit a User 32
- Technical Support 35
- License Agreement 36
- Limitation of Liability 37
- Navigate the Software 38
- Navigating the Software 38
- Image Windows 40
- Fit the Image to the Window 41
- Context Menu Commands 41
- Status Bar 41
- Obtain Image Information 42
- Menu Buttons 44
- Main Menu Buttons 44
- Action Menu Tabs 44
- File Menu Overview 46
- FTP Transfer 50
- Edit Menu Button Overview 52
- Copy an Entire Image 53
- Paste an Image 54
- Paste Special 54
- Undo and Redo 56
- Region of Interest (ROI) 57
- Select a Region 58
- Adjust a Region 58
- Cancel a Region 58
- Image Enhancement Filters 59
- Sharpen 61
- Remove Noise 63
- Adjust the Image 64
- Rotate Function 65
- Align the Image 67
- Flip Image 68
- Resize Image 70
- Reduce Image to Monochrome 71
- Change Image Depth 72
- Advanced Menu Overview 73
- Log Viewer 74
- Display Loaded Modules 79
- Configure Application 80
- Rulers 81
- Using Macros 82
- Edit Macros 83
- Play a User-Defined Macro 83
- Help Menu Overview 84
- Display Help Topics 85
- Application Information 86
- Acquire Images 87
- Image Acquisition 89
- Emission Filters 91
- Magnification 92
- Fine Focus 93
- Bookmarks 93
- Lighting/Darkroom/Lens 95
- Using the BioLite Functions 95
- Darkroom Control 97
- Lens Control 99
- Camera Functions 100
- Exposure Time 101
- Post Processing 102
- Binning 102
- Saturation Preview 102
- Preferences 102
- CCD Temperature 103
- Camera Integration 104
- Dynamic Integration 106
- Binning Modes 107
- Perform 1D Analysis 108
- Finding Lanes and Bands 111
- Lane and Band Information 114
- Add Lanes and Bands 117
- Delete Lanes and Bands 120
- Edit Lanes and Bands 121
- Place Bands Exactly 122
- Lane Profile Overview 124
- Overview 126
- Calibrate a Lane 127
- Stretch Factor 128
- Manual Placement of Weights 129
- Exact Placement of Bands 129
- Background Correction 132
- Joined Valleys 133
- Rolling Disc 134
- Area Between Lanes 134
- Lane Profile Graph 136
- Axis Options 137
- Display Options 137
- Display Options for Details 138
- Selecting Background Color 138
- Concentration Calibration 140
- Change Unit Type 141
- Select Unit Type 141
- Select Data Points 142
- Select Curve Type 143
- Adjust an Automatic Rf Line 145
- Add Rf Lines Manually 146
- Move Rf Lines 146
- Delete Rf Lines 146
- Remove All Rf Lines 147
- Dendrogram Analysis 148
- Generate Dendrogram Graphs 148
- Modify Dendrogram Graphs 149
- Dendrogram Standards 149
- Create Clusters 150
- Linkage Rules 151
- 1D Analysis Settings 153
- Results 155
- Results Data Explorer 156
- Print Data Explorer Reports 157
- Report Types 158
- Perform Area Density 163
- Modify Regions in the Image 165
- • Define Image Scale 166
- Area Density Settings 167
- Define Background 168
- Delete Background Region 169
- Estimate Region Volume 170
- Calibration Curves 172
- Types of Calibration Curves 173
- Save the Results 178
- Print the Results 178
- Export the Results 178
- Perform Colony Counting 179
- Colony Counting Settings 181
- Colony Marking 182
- Label Color 182
- Circle Radius 182
- Counting Colonies - Options 183
- Automatic Counting 184
- Click and Add Counting 186
- Add Colonies 193
- Delete Colonies 194
- Split Colonies 195
- Auto Split Colonies 196
- Merging Colonies 197
- Identify by Color Counting 198
- Classes 200
- Spiral Counting 205
- Adjust Grid Size and Volume 206
- User Tips 208
- Zone Analysis 209
- Colony Counting Reports 210
- Exporting to Excel 211
- Printing Colony Results 211
- Modify Images 218
- Image Action Tab Overview 218
- Image Corrections 219
- Change Brightness 220
- Change Contrast 220
- Change Gamma 221
- Invert Image 221
- Histogram Controls 222
- Pseudocolor 224
- 3D Plots 226
- Viewpoint Tab 227
- Output Tab 227
- Annotations 229
- Annotation Tools 230
- Create Annotations 231
- Create Line Annotations 233
- View or Hide Annotations 235
- Modify Annotations 236
- Rotate Text Annotations 237
- Format Annotations 237
- Delete Annotations 238
- Synchronize Annotations 239
- Measurement Tools 240
- Angle Measure 241
- Layer Actions 242
- Multi-Image Actions 243
- Image Compositing 244
- Player (Image Sequences) 245
- Merge Functions 246
- Player Features 247
- Player Options 247
- Saving .AVI Files 249
- Create Templates 250
- Edit a Template 252
- Synchronize a Template 252
- Delete a Template 252
- Open and Save Images 253
- Image File Types 254
- Print Reports 256
- File Print Command 257
- Print Image History 258
- Print an Image 259
- Purpose 260
- Features 260
- Image History 263
- Glossary 264
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