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Uvp Life Science Manual de usuario Pagina 2
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Life Science Software
1
Table of Contents
3
LS Software User Guide
13
Welcome
15
Capturing Images
16
Minimum System Requirements
17
Registering the Software
18
Offline Activation
19
Single-User License
21
Network-User License
22
Additional Network Users
26
User Administration
28
Users Rights
30
Configure User Accounts
31
Add a New User
32
Edit a User
32
Technical Support
35
License Agreement
36
Limitation of Liability
37
Navigate the Software
38
Navigating the Software
38
Image Windows
40
Fit the Image to the Window
41
Context Menu Commands
41
Status Bar
41
Obtain Image Information
42
Menu Buttons
44
Main Menu Buttons
44
Action Menu Tabs
44
File Menu Overview
46
FTP Transfer
50
Edit Menu Button Overview
52
Copy an Entire Image
53
Paste an Image
54
Paste Special
54
Undo and Redo
56
Region of Interest (ROI)
57
Select a Region
58
Adjust a Region
58
Cancel a Region
58
Image Enhancement Filters
59
Sharpen
61
Remove Noise
63
Adjust the Image
64
Rotate Function
65
Align the Image
67
Flip Image
68
Resize Image
70
Reduce Image to Monochrome
71
Change Image Depth
72
Advanced Menu Overview
73
Log Viewer
74
Display Loaded Modules
79
Configure Application
80
Rulers
81
Using Macros
82
Edit Macros
83
Play a User-Defined Macro
83
Help Menu Overview
84
Display Help Topics
85
Application Information
86
Acquire Images
87
Image Acquisition
89
Emission Filters
91
Magnification
92
Fine Focus
93
Bookmarks
93
Lighting/Darkroom/Lens
95
Using the BioLite Functions
95
Darkroom Control
97
Lens Control
99
Camera Functions
100
Exposure Time
101
Post Processing
102
Binning
102
Saturation Preview
102
Preferences
102
CCD Temperature
103
Camera Integration
104
Dynamic Integration
106
Binning Modes
107
Perform 1D Analysis
108
Finding Lanes and Bands
111
Lane and Band Information
114
Add Lanes and Bands
117
Delete Lanes and Bands
120
Edit Lanes and Bands
121
Place Bands Exactly
122
Lane Profile Overview
124
Overview
126
Calibrate a Lane
127
Stretch Factor
128
Manual Placement of Weights
129
Exact Placement of Bands
129
Background Correction
132
Joined Valleys
133
Rolling Disc
134
Area Between Lanes
134
Lane Profile Graph
136
Axis Options
137
Display Options
137
Display Options for Details
138
Selecting Background Color
138
Concentration Calibration
140
Change Unit Type
141
Select Unit Type
141
Select Data Points
142
Select Curve Type
143
Adjust an Automatic Rf Line
145
Add Rf Lines Manually
146
Move Rf Lines
146
Delete Rf Lines
146
Remove All Rf Lines
147
Dendrogram Analysis
148
Generate Dendrogram Graphs
148
Modify Dendrogram Graphs
149
Dendrogram Standards
149
Create Clusters
150
Linkage Rules
151
1D Analysis Settings
153
Results
155
Results Data Explorer
156
Print Data Explorer Reports
157
Report Types
158
Perform Area Density
163
Modify Regions in the Image
165
• Define Image Scale
166
Area Density Settings
167
Define Background
168
Delete Background Region
169
Estimate Region Volume
170
Calibration Curves
172
Types of Calibration Curves
173
Save the Results
178
Print the Results
178
Export the Results
178
Perform Colony Counting
179
Colony Counting Settings
181
Colony Marking
182
Label Color
182
Circle Radius
182
Counting Colonies - Options
183
Automatic Counting
184
Click and Add Counting
186
Add Colonies
193
Delete Colonies
194
Split Colonies
195
Auto Split Colonies
196
Merging Colonies
197
Identify by Color Counting
198
Classes
200
Spiral Counting
205
Adjust Grid Size and Volume
206
User Tips
208
Zone Analysis
209
Colony Counting Reports
210
Exporting to Excel
211
Printing Colony Results
211
Modify Images
218
Image Action Tab Overview
218
Image Corrections
219
Change Brightness
220
Change Contrast
220
Change Gamma
221
Invert Image
221
Histogram Controls
222
Pseudocolor
224
3D Plots
226
Viewpoint Tab
227
Output Tab
227
Annotations
229
Annotation Tools
230
Create Annotations
231
Create Line Annotations
233
View or Hide Annotations
235
Modify Annotations
236
Rotate Text Annotations
237
Format Annotations
237
Delete Annotations
238
Synchronize Annotations
239
Measurement Tools
240
Angle Measure
241
Layer Actions
242
Multi-Image Actions
243
Image Compositing
244
Player (Image Sequences)
245
Merge Functions
246
Player Features
247
Player Options
247
Saving .AVI Files
249
Create Templates
250
Edit a Template
252
Synchronize a Template
252
Delete a Template
252
Open and Save Images
253
Image File Types
254
Print Reports
256
File Print Command
257
Print Image History
258
Print an Image
259
Purpose
260
Features
260
Image History
263
Glossary
264
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