Uvp Life Science Manual de usuario descargar pdf (Pagina 148)

LS Software User Guide

134

Dendrogram Analysis

Performing Dendrogram Analysis

• Overview

• Generate Dendrogram Graphs

• Modify Dendrogram Graphs

• Dendrogram Standards

• Create Clusters

Overview

Dendrograms are graphical/numerical displays used to show the similarity of bands. The similarity is

grouped into clusters. Each lane in an image has its own cluster. Then, repeatedly, a linkage rule

(selected by the user in the software) is employed to merge smaller groups into larger clusters, until all

the clusters have been combined into a single cluster. The result is a hierarchy of clusters. When moving

up the hierarchy, each cluster contains more but less similar lanes. Lanes that are very similar to each

other will appear together in clusters near the bottom of hierarchy.

This section will explain how to:

• Automatically generate dendrogram information

• Automatically generate multi-image dendrogram information

• Modify dendrogram graphs

Generate Dendrogram Graphs

After finding the lanes and bands in the image the user may begin the process of generating dendrogram

graphs.

• Finding lanes and bands must be performed first. (See: Finding and Modifying Lanes and Bands)

• Go to 1D Analysis > Dendrogram.

• If only one image is open, a Dendrogram View window will appear.

• The user may select from Band Formula: Rf Values or Weight Values, Lane Formula: Dice

Similarity or Jaccard Similarity and the Linkage Formula.

• A Dendrogram View Graph shows the relationships between lanes and bands.

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Uvp Life Science Manual de usuario Pagina 148