Uvp Life Science Manual de usuario descargar pdf (Pagina 137)

Perform 1D Analysis

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Axis Options

By default, the x-axis displays Pixels and the y-axis displays Intensity. However, after calibrating

molecular weight, Rf values or Molecular Weights (MW Standard) may be a better naming option for the

x-axis. Similarly, after calibrating concentration, Concentration may be a better naming option for the y-

axis.

Change Axis Units

• To change axis units after performing either molecular weight calculations or concentration

calibrations, simply go to the Y Axis and X Axis options under the bottom left of the graph. Use

the drop down menus to select the desired variable.

• If Retardation factor (Rf) or Molecular Weight (MW) is selected to be displayed on the x-axis, then

the graph takes into account Rf effects. This means that other lanes may appear to be stretched

or compressed horizontally relative to the selected lane.

• Once Concentration is selected to be displayed on the y-axis, the curve adjusts the intensities of

the lane, and the relative differences in the graph may change.

Display Options

In the Lane Profile graph, the software allows users to choose what details to show in the graph. The

program also allows users to change the colors of the background, graph, and axes.

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Uvp Life Science Manual de usuario Pagina 137